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Thin Layer Chromatography
Automatic Developing System
THE
TIPPER
The
TIPPER automatic developing system is a revolutionary advance in
the development of TLC plates.
The TIPPER consists of a
computerized control module with TLC tank platform.
After placing a TLC plate in
the tank, the unit will automatically equilibrate and develop the plate to the
parameters set by the user.
ADVANTAGES
Because TLC is a manual technique, inconsistencies from
technician to technician can often result in imprecise results. The proper and
consistent development of TLC plates has long been a concern of
chromatographers. Until now, manual methods of developing TLC plates were the
only way to achieve separation.
The problem with manual development methods is that they
often promote uneven solvent fronts due to "waves" that are created by
the placement of the plate into the tank. Often this wave so badly distorts the
solvent front that the chromatographer is forced to start all over again wasting
valuable time and supplies.
The TIPPER eliminates
uneven solvent fronts by assuring a smooth, consistent and reproducible
transition of the TLC plate into the mobile phase.
Some separations require or are enhanced by
"equilibration" of the developing chamber prior to the beginning of
development. Several cumbersome tanks are sold which permit the TLC plate to be
suspended above the mobile phase during equilibration. Then the plate is lowered
into the mobile phase by means of special rods or levers.
The TIPPER eliminates the need for any special equilibration apparatus by
incorporating a built-in "count-down" timer that lets the user specify
an equilibration time prior to development. Again, this results in consistent
and reproducible results.
Over development of TLC plates is one of the most frustrating
aspects of the technique. Chromatographers often complain of accidentally
allowing the solvent front to run "over" the top of the plate thus
eliminating the possibility of calculating Rf values.
The TIPPER prevents over-development by automatically stopping
the development of the TLC plate and signaling the technician with an audible
alarm.
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